Ethylacetate Extract Of Annona Squamosa Seeds Containing Anti-Head Lice Activity
The present study focused on the separation and identification of the active compounds against head lice from the ethyl acetate extract of Annona squamosa L seed. Chromatographic and spectroscopic techniques revealed that two major compounds of the hexane seed extract were oleic acid and triglyceride with one oleate ester. The yields of these compounds were 13.88% and 7.70% dry weight, respectively. The compounds were tested in vitro against head lice, comparing to the crude ethyl acetate extract of the seed. The triglyceride with one oleate ester and the crude ethyl acetate extract diluted with coconut oil 1:1. These compounds were found to kill all tested head lice in 10 and 31 minutes, respectively. The triglyceride ester can be used as a marker for quantitative analysis of the active compound for quality control of the raw material A. squamosa seed and its extract. This finding will be useful for quality assessment and the chemical stability of the anti-head lice preparation from this plant.