TY - JOUR T1 - Simultaneously Extraction of Caffeine and Chlorogenic Acid from Coffea canephora Bean using Natural Deep Eutectic Solvent-Based Ultrasonic Assisted Extraction JF - Pharmacognosy Journal Y1 - 2019 A1 - Adisya Miftah Syakfanaya A1 - Fadlina Chany Saputri A1 - Abdul Mun’im KW - Caffeine KW - Chlorogenic acid KW - Coffea canephora KW - Natural deep eutectic solvent KW - Ultrasonic assisted extraction AB -

Background: NADES is an alternative solvent in the extraction of metabolites from plants which has many environmental benefits, such as low toxicity, biodegradability, can dissolve polar and non-polar compounds, low costs and simple preparation. Objective: This study aims to determine the effect of natural deep eutectic solvent-based ultrasonic-assisted extraction (NADES-UAE) on enrichment of caffeine and chlorogenic acid in extract from green coffee beans (Coffea canephora). Methods: The powders were extracted using NADES-UAE method in several types of extraction condition, including the composition of NADES, water addition in NADES and extraction time. Caffeine and chlorogenic acid content were analyzed using HPLC, reverse phase system and C18 ODS-3 column. Results: The highest of caffeine and chlorogenic acid content was respectively, 7.89 mg/g and 28.62 mg/g (composition of NADES betaine: sorbitol [1:1.2] ratio and NADES-water addition [1:2] ratio for 30 min). Conclusion: This research showed that the composition of NADES, extraction time and water addition are important parameter in extracting caffeine and chlorogenic acid content in green coffee beans.

VL - 11 IS - 2 ER -