TY - JOUR T1 - Inhibitory effect of Bryophyllum pinnatum (Lam.) Oken leaf extract and their fractions on α-amylase, α-glucosidase and cholinesterase enzyme JF - Pharmacognosy Journal Y1 - 2018 A1 - Oluwafemi Adeleke Ojo A1 - Adebola Busola Ojo A1 - Basiru Olaitan Ajiboye A1 - Oluranti Olaiya A1 - Ayodeji Akawa A1 - Oyindamola Olaoye A1 - Omosola Olufisayo Anifowose A1 - Olajumoke Idowu A1 - Oluwaseun Olasehinde A1 - Tajudeen Obafemi A1 - Joseph Awe A1 - Babatunji Emmanuel Oyinloye KW - Antioxidants KW - Bryophyllum pinnatum (Lam.) KW - Cholinesterase KW - Enzyme inhibitory activity KW - Oken AB -

Bryophyllum pinnatum (Lam.) Oken leaves are employed as food and as traditional medicines. This study investigates the antioxidant activity (reducing power, DPPH, ABTS, FRAP, H2O2 scavenging ability and metal ion chelating), carbohydrate digesting enzymes activity and inhibitory activity of cholinergic enzyme of aqueous extract and fractions (n-hexane, ethyl acetate, n-butanol, residual aqueous fraction) of B. pinnatum leaves were investigated. Results showed that aqueous extract of B. pinnatum exhibited DPPH radical scavenging abilities, iron chelation, hydrogen peroxide scavenging abilities and reducing power (Fe3+- Fe2+). B. pinnatum aqueous extract also had considerably α-amylase and α-glucosidase inhibitory activities with IC50 values 149.20 ± 14.44 μg/mL and 126.15 ± 9.76 μg/mL respectively. Our findings indicated that ethyl acetate fraction contained a considerably higher (p < 0.05) amount of total phenolic, flavonoids, total antioxidant, FRAP, metal ion, ABTS and DPPH radical scavenging activity than other solvent fractions. Furthermore, the ethyl acetate fraction elicited a significantly higher (p < 0.05) inhibitory effects on α-glucosidase (IC50 = 70.90 ± 1.23 μg/ml), α-amylase (IC50 = 62.45 ± 1.22 μg/ml), acetylcholinesterase (AChE) (IC50 = 66.75 μg/mL) and butyrylcholinesterase (BChE) (IC50 = 62.97 μg/mL) activities than other fractions. Hence, B. pinnatum leaves were rich in biologically active components; thus, could be employed to formulate new plant-based pharmaceutical and nutraceutical drugs to improve human health.

VL - 10 UR - http://fulltxt.org/article/515 IS - 3 ER -