02139nas a2200337 4500008004100000245006600041210006300107260001800170300000900188490000600197520120200203653001601405653001701421653001201438653000901450653001001459653001801469653002601487653001901513653001001532653003901542653001301581653001701594653002201611100003101633700002101664700002801685700002201713700002801735856003801763 2017 eng d00aCytotoxic Compounds from Kibatalia gitingensis (Elm.) Woodson0 aCytotoxic Compounds from Kibatalia gitingensis Elm Woodson cDecember 2016 a8-130 v93 a
Ursolic acid (1), squalene (2), a mixture of α-amyrin acetate (3a) and lupeol acetate (3b), and isoscopoletin (4), isolated from the dichloromethane extracts of the leaves and twigs of Kibatalia gitingensis, were evaluated for their cytotoxic activities against three human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast-neonatal (HDFn), using the in vitro PrestoBlue® cell viability assay. Compounds 1-4 exhibited strong cytotoxic activities against HT-29 cells with IC50 values ranging from 0.6931 to 1.083 μg/mL. Furthermore, 1-4 were moderately cytotoxic against HCT-116 cells with IC50 values ranging from 4.065 to 11.09 μg/mL. These compounds were least cytotoxic against MCF-7 cells with IC50 values ranging from 8.642 to 25.87 μg/mL. The most cytotoxic against HT-29 cells, HCT-116 cells and MCF-7 cells are 2, 4 and 1, respectively.
10aApocynaceae10aCytotoxicity10aHCT-11610aHDFn10aHT-2910aIsoscopoletin10aKibatalia gitingensis10aLupeol acetate10aMCF-710aPrestoBlue® cell viability assay.10aSqualene10aUrsolic acid10aα-amyrin acetate1 aReyes, Mariquit, M. De Los1 aOyong, Glenn, G.1 aNg, Vincent, Antonio S.1 aShen, Chien-Chang1 aRagasa, Consolacion, Y. uhttp://www.phcogj.com/article/218