@article {1066, title = {Chemical Fingerprint by HPLC-DAD-ESI-MS, GC-MS Analysis and Anti-Oxidant Activity of Manasamitra Vatakam: A Herbomineral Formulation}, journal = {Pharmacognosy Journal}, volume = {12}, year = {2020}, month = {February 2020}, pages = {115-123}, type = {Research Article}, chapter = {115}, abstract = {

Background: Manasamitra Vatakam is a classical ayurvedic herbo mineral formulation used for the treatment of neurodegerative properties and epileptic disorders. The wide range mixture of herbal extracts and minerals were used in the formulation. Aim: The aim of the study implies in performing the chemo-profiling, chromatographic fingerprint analysis by HPLC-DAD-ESI-MS for the selected formulations of Manasamitra Vatakam followed by the identification of bioactive compounds by Gas Chromatography {\textendash} Mass Spectrometric (GC-MS) analysis, to evaluate the diffusion and dilution methods for the determination of anti-bacterial activity in the methanolic extracts of Manasamitra Vatakam (MMV). Materials and Methods: The antibacterial activity was performed by both diffusion and dilution methods whereas the antioxidant activity was performed by free radical scavenging of 2,2-diphenyl-1-picrylhydrazy and hydrogen peroxide scavenging assay method. Results: The estimation of bioactive constituents showed positive results by qualitative analysis. Antibacterial activity of MMV was evaluated against two-gram positive Staphylococcus aureus and Bacillus cereus, two gram negative Escherichia coli and Klebsiella pneumonia by disk diffusion (0.078-10μg mL-1), broth dilution (0.078-10μg mL-1) and broth micro dilution method (0.39-50μg mL-1) respectively. The bioactive constituents were analysed by GC-MS analysis for the methanolic extract of the formulation. Conclusion: To conclude, the formulation was found abundant with phenolic and flavonoid compounds by HPLC-ESI-MS analysis, the bioactive compounds identified are responsible for the anti-bacterial activity. The broth microdilution method performed by resazurin method was observed as the fast screening, sensitive and accurate method for the quantitative determination of antibacterial activity.

}, keywords = {Classical formulation, Diffusion and dilution methods, Heavy metals, MIC, Phytochemicals}, doi = {10.5530/pj.2020.12.18}, author = {Srikalyani V and Ilango K} }