@article {1831, title = {The Effect of Omega-3 Rich Fish Oil on the Kidney Changes in Mice Induced by Azoxymethane and Dextran Sodium Sulfate}, journal = {Pharmacognosy Journal}, volume = {14}, year = {2022}, month = {August 2022}, pages = {259-266}, type = {Original Article }, chapter = {259}, abstract = {

Background: The study aimed to investigate the effect of omega-3 rich fish oil to kidney of mice induced by Azoxymethane (AOM) and DSS using histopathology parameters. Method: The experimental mice were induced using 10 mg/kg AOM and 2\% DSS for 2 weeks randomly allocated randomly into four groups as follows; Control Group: mice that not received fish oil, Low Dose Group: mice that received 1.5 mg/day fish oil, Medium Dose Group: mice that received 3 mg/day fish oil, and High Dose Group: mice that received 6 mg/day fish oil. The omega-3 rich fish oil was given for 12 weeks. Result: The administration of high dose omega-3 rich fish oil was able to reduced necrosis and inflammation foci compared to the control group (p\<0.05). Furthermore, the administration of low, medium, and high dose omega-3 rich fish oil was able to significantly reduced vascular edema and cell degeneration foci (p\<0.05). The administration of medium and high dose of omega-3 rich fish oil were able to reduce the amount of fibrosis foci compared to the control group (p\<0.05) compared to the control group. Conclusion: The result suggested anti-nephrotoxic effect of omega-3 rich fish oil in mice induced by azoxymethane and DSS.

}, keywords = {Azoxymethane, Dextran sodium sulfate, Fish oil, Histopathology, Kidney, Mice, Omega-3}, doi = {10.5530/pj.2022.14.94}, author = {Kusmardi Kusmardi and Paulus Anthony Halim and Wachid Putranto and Aryo Tedjo} } @article {625, title = {In silico, in vitro and in vivo Tests of Ficus deltoidea Jack Leaves Extract as Inhibitor for Beta-Catenin Expression in Colon Carcinogenesis Model}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {June 2018}, pages = {808-813}, type = {Original Article}, chapter = {808}, abstract = {

Context: Ficus deltoidea Jack leaves extract as anticolorectal cancer. Aims: This study aims to analyze the potential of FD extract to be an anti-colon cancer by investigating the extract capability in reducing \β-catenin expression and inhibiting colon cancer cells growth. Settings |and Design: The research was conducted in Medical Faculty Universitas Indonesia with experimental design. Methods and Material: FD ethanol extracts was tested in vitro, in silico and in vivo. In vitro test was conducted to human colon cell lines. In vivo test was conducted to Balb/c mice induced with 10 mg/kg azoxymethane (AOM) and dextran sodium sulfate 1\% (DSS). The colonic tissue collected was the distal portion. \β-catenin expressions in the cytoplasm and nuclei of the epithelial cells of the colon crypt were semi quantitatively assessed using the immunohistochemistry staining on ten visual fields with 400x magnification. Statistical analysis used: SPSS. Results: FD ethanol extracts inhibit the expression of \β-catenin in the crypt ephitelial cells of mice colon induced with AOM/DSS. The extracts also inhibit the growth of human colon cancer (HCT 116) with IC50 value of 5.41 mg/mL. Phytochemical screening to the extracts gave three groups of compounds: alkaloid, flavonoid, and tannin. Water fraction is the best fraction. Based on in the results of in silico analysis with molecular docking, FD extract is believed to influence the expression of \β-catenin, in which vitexin and isovitexin are the main candidate compounds to influence the expression of the protein. Conclusion: FD ethanol extract is potential to be an anti-colon cancer proven by the extract capability to reduce \β-catenin expression.

}, keywords = {Azoxymethane, Colon carcinogenesis, Ficus deltoidea, in silico, β-catenin}, doi = {10.5530/pj.2018.4.137}, url = {http://fulltxt.org/article/675}, author = {Kusmardi Kusmardi and Tedjo Aryo and Wuyung Puspita Eka and Fadilah Fadilah and Priosoeryanto Bambang Pontjo and Fachri Wilzar} }