@article {635, title = {Quantitative and Optimization of Anthocyanin Extracted from Pomegranate (Punica Granatum) Extract by High-Performance Liquid Chromatography (HPLC)}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {June 2018}, pages = {650-653}, type = {Original Article}, chapter = {650}, abstract = {

Objective: P. granatum is one of the oldest edible fruits of tropical and subtropical regions. This fruit had high antioxidant contained by hydrolysable tannins and anthocyanin compounds that give many health benefit properties. This study aims to quantify and optimized anthocyanin from P. granatum extract. Methods: A total of 50g of the flesh was soaked into two different polar solvents; water and 50\% ethanol within a ratio of 1:10; w/v for 24-hr. Then, three different methods of extraction were done and test each with HPLC analytical, respectively. Results: The validated method proved to be linear in the range of 5 \– 30 ug/mL and with LOD and LOQ determined respectively for Cy3, Cy3, 5, Pg3, and Pg3, 5. The method also shows recovery (\%) close to 100 when accuracy was accessed. For samples, blender water extract had a higher composition of Cy3, Cy3, 5 and Pg3, 5 (22.77 \± 8.82 mg/100 g e.p; 25.36 \± 9.95 mg/100 g e.p; 11.16 \± 5.85 mg/100 g e.p) content as compared to other. Conclusion: As a conclusion, the present methodology proved to be capable of detecting and quantifying Cy3, Cy3, 5, Pg3, Pg3, 5 in a single run. Also, comparatively the composition of each AC detected in blender water extract is significantly higher in value than the other methods. It should regard as a valuable source of antioxidant with the potential used for health benefits properties worldwide.

}, keywords = {5-diglucoside, Cyanidin 3, Cyanidin 3-glucoside, Pelargonidin 3, Pelargonidin 3-glucoside}, doi = {10.5530/pj.2018.4.107}, url = {http://fulltxt.org/article/645}, author = {Ridzwan Norhaslinda and Jumli Mimie Noratiqah and Baig Atif Amin and Rohin Mohd Adzim Khalili} } @article {693, title = {Quantitative and Optimization of Phenolic Acid Extracted from Pomegranate by High Performance Liquid Chromatography (HPLC)}, journal = {Pharmacognosy Journal}, volume = {10}, year = {2018}, month = {August 2018}, pages = {969-972}, type = {Original Article}, chapter = {969}, abstract = {

Objective: Pomegranate is scientifically known as Punica granatum L. which is a nutrient dense fruit rich in phytochemical compounds. Phenolic content is the main compound attribute for the most of the functional properties in pomegranate. The aim of this study is to quantify and optimize the composition of phenolic acids extracted from pomegranate extract by using High-performance Liquid Chromatography (HPLC). Method: The pomegranate extracted with three different methods by using two different solvents which is 50\% ethanol and water. The methods were blended (aril+ seed), Soaking (aril+ seed) and soaking + squeezed manually. HPLC-PDA was used as equipment to quantify and optimize the phenolic acids extracted from pomegranate. Result: Validation method of HPLC was analysed according to the percentage of recovery, LOD, LOQ and coefficient correlation. Result showed that GA was detected in all sample from different method of extraction applied while CA compound not detected in any of extraction method applied. FA compound was only detected in blended method by 50\% ethanol and water as solvent while EA compound was detected only in water extraction of all three methods applied. Conclusion: As a conclusion, according to the standard calibration data curve showed that this method proved to detect and quantify the targeted compounds. By comparing the data obtained from this study, it showed that water blended extract method is significantly higher content of targeted compound except for the CA compound. To the best of our knowledge, this sample can be a valuable source of antioxidant for better used in health benefits.

}, keywords = {Caffeic acid, Ellagic acid, Ferulic acid, Gallic acid, HPLC, Pomegranate extract}, doi = {10.5530/pj.2018.5.164}, author = {Jumli Mimie Noratiqah and Ridzwan Norhaslinda and Baig Atif Amin and Rohin Mohd Adzim Khalili} } @article {403, title = {Cytotoxicity Effect and Morphological Study of Different Duku(Lansium domesticum corr.) Extract towards Human Colorectal Adenocarcinoma Cells Line (HT-29)}, journal = {Pharmacognosy Journal}, volume = {9}, year = {2017}, month = {September 2017}, pages = {757-761}, type = {Original Article}, chapter = {757}, abstract = {

Context: Lansium domesticum corr. is a member of the family Meliaceae, and known locally as duku and has been used traditionally in the prevention and treatment of various illness. Aim: To study the cytotoxic effect and morphological changes of human colorectal adenocarcinoma cells (HT-29) treated with different duku (Lansium domesticum corr.) extracts. Methods: The L.domesticum corr. fruit extracts were processed involving three different solvents; methanol, ethanol and ethyl acetate. HT-29 cell lines were treated with different concentrations of L. domesticum corr. (0-100 \μg/ml) extracts for a total of 24, 48 and 72 hours. Cytotoxicity of cells line was determined by using MTT assay as per IC50 values. Results: Methanol extract of L. domesticum corr. showed IC50 value at 6.79 \± 0.00 \μg/ml and 50.0 \± 0.00 \μg/ml respective, while ethyl acetate extract of L. domesticum corr. reached IC50 value at 86.00 \± 0.08 \μg/ml, and 96.0 \± 0.12 \μg/ml. There was no IC50 value of ethanol extract from L.domesticum corr. Only methanol extract showed toxicity towards HT-29 cells line. Conclusion: To the best of our knowledge, this is the first repeat the exploring the effect of duku (L. domesticum corr.) extract on HT-29 cells line.

}, keywords = {Anti-proliferative effect, colorectal cancer, IC50 value., MTT Assay, polarity extracts}, doi = {10.5530/pj.2017.6.119}, url = {http://fulltxt.org/article/172}, author = {Rohin Mohd Adzim Khalili and Jumli Mimie Noratiqah and Ridzwan Norhaslinda and Abd Hadi Norhayati and Baig Atif Amin and Arshad Roslan and A. Latif Ahmad Zubaidi} }